CFX96TOUCH Fluorescent Quantitative PCR qalab

CFX96Touch fluorescent quantitative PCR waxaa loo isticmaali karaa noocyo kala duwan oo cilmi baaris ah oo ku saabsan qiyaasaha aashitada nucleic, falanqaynta heerka muujinta hidda-wadaha, ogaanshaha hidda-wadaha, ogaanshaha GMO, iyo falanqaynta gaarka ah ee sheyga.

Deegaanka Shaqada:
1.1 Heerkulka shaqada: 5-31 ° C
1.2 Shaqada iyo qoyaanka: qoyaan qaraabo ≤80%
1.3 Awoodda shaqada: 100-240 VAC, 50-60Hz
Waxqabadka iyo shuruudaha farsamada ee CFX96Touch fluorescence quantitative PCR
3.1 Waxqabadka ugu weyn (* waa tilmaame ay tahay in la buuxiyo)
* 3.1.1 Lix kanaal oo tijaabo ah, 5% PCR waa la xaqiijin karaa, iyo 5 hidde-sideyaasha bartilmaameedka ah ayaa la ogaan karaa isku mar, kanaalka ogaanshaha FRET ee gaarka ah ayaa isku mar la ogaan karaa.
* 3.1.2 Iyada oo la adeegsanayo PCR heerkulka firfircoon, waxaad ku socon kartaa 8 heerkul kala duwan isku mar, heerkul kasta oo kuleej ah.
3.1.3 Furitaanka reagents dhamaystiran, cilmi baaris kala duwan iyo reagents bukaan ayaa codsanaya;
3.1.4 Ku haboon noocyada kala duwan ee hababka fluorescence sida Taqman, Molecular Beacon, Fret probe, Sybr Green i, iwm .;
3.1.5 Furan, 0.2ml hal tube, octal, saxan 96-ceel ah, iwm.
* 3.1.6 waxay u shaqayn kartaa si madax banaan, oo run ahaantii ka shaqaynaysa offline, looma baahna in lagu xidho kombayutarka si loo kormeero qalooca korantada ee PCR wakhtiga dhabta ah;
3.2 Shuruudaha farsamada ee ugu muhiimsan (* waa tilmaame ay tahay in la buuxiyo)
* 3.2.1 Awood muunad: 96 × 0.2ml, qeexitaannada caadiga ah 96-ceel saxan (12 × 8) ayaa la isticmaali karaa;
3.2.2 Nooca Sahayda: 0.2ml hal tuubo, siddeed isku xiran, 96-ceel saxan, iwm.
3.2.3 Nidaamka Falcelinta: 1-50μL (lagu taliyay 10-25 μL);
* 3.2.4 Isha iftiinka: Lix LED oo leh filtarrada;
* 3.2.5 Baaraha: Lix diimood oo sawir leh oo leh filtarrada;
* 3.2.6 litir xawaaraha qaboojinta: 5 ° C / sek;
3.2.7 Xakamaynta heerkulka: 0 -100 ° C;
3.2.8 Saxnaanta heerkulka: ± 0.2 ° C (90 ˚C);
3.2.9 Isku mid ahaanshaha heerkulka: ± 0.4 ° C (90 ˚C gudaha 10 ilbiriqsi);
* 3.2.10 Shaqada Gradient Heerkulka Firfircoon: Ku wad 8 heerkul kala duwan isku mar; heerkulka xakamaynta tartiib tartiib ah: 30 -100 ° C; kala duwanaanshaha heerkulka tartiib tartiib ah: 1 - 24 ° C; waqtiga kulaylinta heerkulka gradient: isku mid;
3.2.11 xamaasad / qulqulka hirarka dhererka: 450-730 nm;
3.2.12 Dareenka: Hal koob oo hidde-side ah oo ku jira genome-ka aadanaha waa la ogaan karaa;
3.2.13 Kala duwanaanshaha firfircoon: 10 tiro;
3.2.14 Bandhig: 8.5 inch midabka taabashada;
3.2.15 Habka Falanqaynta Xogta: Heerka Curve Quantity, Curve Melting, CT ama ΔΔCT Falanqaynta Muujinta Gene, Falanqaynta Genodes-ka Gudaha ee Badan iyo Xisaabinta Waxtarka, Faylasha Xogta Badan ee Falanqaynta Muujinta Hida, Falanqaynta Allelic, Falanqaynta Nooca, Hayso Hid, shaqada falanqaynta qalooca ;
3.2.16 Dhoofinta Xogta: Excel, Word, ama PowerPoint. Warbixinta isticmaaluhu waxa ay ka kooban tahay habaynta socodsiinta, garaafyada, iyo natiijooyinka xogta miiska, kuwaas oo loo daabici karo ama loo kaydin karo PDF ahaan;
* 3.2.17 Daraasadaha qaabdhismeedka Chromosomal: Habka falanqaynta tirada ee qaab-dhismeedka chromatin iyadoo la barbardhigayo doorka isbarbardhigga DNA-da genomic iyadoo la barbardhigayo doorka isbarbardhigga DNA-da genomic. Waxay si dhab ah u caddaynaysaa xidhiidhka dhererka u dhexeeya qaab-dhismeedka chromatin iyo muujinta hidda-wadaha;